Protocols
- Drosophila Media Recipes (The Bloomington Drosophila Stock Center, Indiana University)
- Standard medium
- Hard agar medium
- Cornmeal, Sucrose, Dextrose, Yeast and 2-Acid Medium
- Cornmeal-molasses-yeast medium
- Cornmeal-sucrose-yeast medium
- Sucrose-yeast medium
- Cornmeal-dextrose-yeast medium
- Semi-defined medium
http://flystocks.bio.indiana.edu/media-recipes.htm
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Basic Methods of Culturing Drosophila
(The Bloomington Drosophila Stock Center, Indiana University)
Detailed method for culturing fly.
http://flystocks.bio.indiana.edu/culturing.htm
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Culture of Drosophila Cells
(Drosophila RNAi Screening Center at Harvard Medical School)
Covering culture medium, growth conditions, maintainance, freezing and thawing...
http://flyrnai.org/RNAi_FAQ_dros_cc.html
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Current Bloomington Recipe for Drosophila Medium
(The Bloomington Drosophila Stock Center, Indiana University )
Includes the recipe, cooking instructions and ingredients notes.
http://flystocks.bio.indiana.edu/bloom-food.htm
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Cuticle preparations
(Henry M. Krause's Lab,University of Toronto)
http://www.utoronto.ca/krause/cuticles.html
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Dissection of Drosophila CNSs
(ITO Kei, JFly)
Preparation of dissection tools, How to dissect brains of pupae and adults
http://jfly.nibb.ac.jp/html/manuals/pdf/E_CNS_Diss...
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Growing imaginal disc cells
(Nusse Lab, Stanford University)
general maintenance instructions for imaginal disc cell lines
http://www.stanford.edu/~rnusse/ownpage/protdiscce...
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Hard Agar Medium for Egg Collection Plates
(The Bloomington Drosophila Stock Center, Indiana University )
This is a firm medium that keeps well and makes a good base for egg collecting
http://flystocks.bio.indiana.edu/hard-agar.htm
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Methods for the preparation of Fly Extract
(School of Biology, University of St. Andrews)
For primary and routine culture
http://www-sbms.st-and.ac.uk/sites/flycell/flyextr...
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production of wingless protein
(Nusse Lab, Stanford University )
Production of Conditioned Medium containing Wingless protein from S2 cells
http://www.stanford.edu/~rnusse/ownpage/production...
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Routine methods for growing the cell lines
(School of Biology, University of St. Andrews)
Medium/Insulin/Calcium- and magnesium- free saline/Passaging cell lines/Cell seeding numbers
http://www-sbms.st-and.ac.uk/sites/flycell/growing...
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Single-Fly DNA Preparations for PCR
(Lazo Lab)
A simple method for the rapid and reproducible isolation of DNA from single flies for amplification by PCR and direct sequencing by asymmetric PCR.
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafly...
Added: Tue May 14 2002, Hits: 388, Reviews: 0 Write review Cached