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Protocols
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Antibody Staining of Dissected Gonads
( )
http://www.genetics.wustl.edu/tslab/gonad_antibody...
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Antibody Staining (MGH)
(Ambros Lab)
http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
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Basic Immunofluorescence of C. elegans Embryos
(Aroian Lab)
http://www-biology.ucsd.edu/labs/aroian/protocols/...
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C. elegans whole mount immunohistochemistry with Bouin's Fixative
(Nonet Lab - Washington University School of Medicine)
a fixation protocol which provides an alternative set of conditions to test antisera that work poorly on paraformaldehyde fixed worms
http://thalamus.wustl.edu/nonetlab/ResourcesF/fix....
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DAPI Staining
( )
http://www.genetics.wustl.edu/tslab/dapi_staining....
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Double dye fill
(Herman Lab)
http://www.ksu.edu/hermanlab/protocols/double_dye_...
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Dye Filling to Stain Amphid and Phasmid Neurons (Michael Koelle)
(Ambros Lab)
http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
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Dye Filling to Stain Amphid and Phasmid Neurons:
( )
Michael Koelle
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
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EMS mutagenesis
(Herman Lab)
http://www.ksu.edu/hermanlab/protocols/ems_mutagen...
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Fixation & Staining Parameters
(Aroian Lab)
http://www-biology.ucsd.edu/labs/aroian/protocols/...
Added: Tue May 14 2002, Hits: 504, Reviews: 0
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Fixation and Permeabilization
(Mike Finney)
This protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate).
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
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Fixing Individual Embryos--Carolyn Norris
( )
http://www.aecom.yu.edu/wormem/embryo.htm
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Germline In Situ Hybridization
( )
http://www.genetics.wustl.edu/tslab/gonad_in_situ....
Added: Tue May 14 2002, Hits: 387, Reviews: 0
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Gonad Dissections
(Schedl Lab)
http://www.genetics.wustl.edu/tslab/gonad_dissecti...
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Gonad Fixation
( )
http://www.genetics.wustl.edu/tslab/gonad_fixation...
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Hypochlorite Treatment
(Seydoux Lab)
Gental and regular hypochlorite treatment.
http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/hy...
Added: Tue May 14 2002, Hits: 41, Reviews: 0
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in situ Hybridization
(Seydoux Lab)
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/in...
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In situ hybridization
(Herman Lab)
http://www.ksu.edu/hermanlab/protocols/in_situ_hyb...
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In situ hybridization : Geraldine Seydoux and Andy Fire
(Ambros Lab)
http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambro...
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In situ hybridization:
( )
Geraldine Seydoux and Andy Fire
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 60, Reviews: 0
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lacZ Freeze Crack Staining Embryos
(Seydoux Lab)
http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/la...
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Microwave Fixes--David Hall and Agness Miller
( )
http://www.aecom.yu.edu/wormem/micro.htm
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MMS integration
(Herman Lab)
http://www.ksu.edu/hermanlab/protocols/mms_integra...
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Nematode dye filling
(Herman Lab)
http://www.ksu.edu/hermanlab/protocols/nematode_dy...
Added: Tue May 14 2002, Hits: 727, Reviews: 0
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P Granule Staining of Embryos
(Seydoux Lab)
http://www.bs.jhmi.edu/MBG/SeydouxLab/Protocols/PG...
Added: Tue May 14 2002, Hits: 56, Reviews: 0
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