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Protocols
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Bacteria Growth and Culture Bacteria Growth and Culture
(Michael Blaber)
Very useful background knowledge about bacteria growth and replication and very basic guide to bacteria culture techniques. If you are new to bacteria culture, it's a must to read this.
http://wine1.sb.fsu.edu/bch5425/lect11/lect11.htm
Added: Tue May 14 2002, Hits: 3248, Reviews: 0
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Bacteria Storage
(Lazo Lab)
Maintenance of Probes in bacteria including Escherichia coli with stab agar or glycerol method
http://wheat.pw.usda.gov/~lazo/methods/lazo/bactst...
Added: Tue May 14 2002, Hits: 5197, Reviews: 0
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Bacterial cell maintenance
(Roe, OU)
Basic procedures for bacteria culture
http://www.genome.ou.edu/protocol_book/protocol_pa...
Added: Tue May 14 2002, Hits: 8874, Reviews: 0
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Bacterial Glycerol Stocks
(Van Dyke Lab, University of North Carolina)
http://www.unc.edu/depts/van_dyke/protocols/bacter...
Added: Sat Mar 27 2004, Hits: 8708, Reviews: 0
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Bacteriology
(The Morimoto Lab, Northwestern University)
Introduces basic methods of bacteria culture, such as media and plate preparation, growing, streaking and storing bacteria.
http://www.biochem.northwestern.edu/morimoto/resea...
Added: Mon Mar 29 2004, Hits: 10490, Reviews: 0
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Growing Overnight Cultures
(John Roth, Division of Biological Sciences,University of California at Davis)
Growing overnight cultures of single clonies
http://rothlab.ucdavis.edu/protocols/M02.html
Added: Sun Jun 06 2004, Hits: 8061, Reviews: 0
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Isolation of actinomycetes from Soil
(Actinomycetes-Streptomyces Internet Resource Center)
http://www.cbs.umn.edu/asirc/protocol/isolation.ht...
Added: Fri Sep 27 2002, Hits: 492, Reviews: 0
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Long Term Storage of Bacterial Strains
(Donis Keller Lab)
Bacterial strains may be stored indefinitely at low temperatures (- 20 degrees C and -80 degrees C) in 15 to 40% glycerol.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
Added: Tue May 14 2002, Hits: 3820, Reviews: 0
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Lyophilizing Cells
(Goldberg Lab)
http://wheat.pw.usda.gov/~lazo/methods/goldberg/ly...
Added: Tue May 14 2002, Hits: 1214, Reviews: 0
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Preparing Overnight Bacteria Culture
(LaboratoryExperiments.com)
This is a basic procedure for high school students and useful for those who are new to molecular biology.
http://www.laboratoryexperiments.com/LAB5.htm
Added: Tue May 14 2002, Hits: 6138, Reviews: 0
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Replica Printing
(John Roth, Division of Biological Sciences,University of California at Davis)
http://rothlab.ucdavis.edu/protocols/M05.html
Added: Sun Jun 06 2004, Hits: 1404, Reviews: 0
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Streaking Bacteria for Single Colonies
(John Roth's laboratory, UC Davis)
http://rothlab.ucdavis.edu/protocols/M01.html
Added: Wed Oct 16 2002, Hits: 5422, Reviews: 0
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Streaking Bacterial Stocks
(Donis Keller Lab)
Bacterial cells are streaked onto a medium to obtain an independent isolate. This is done to reduce the likelihood of working with a culture which has become contaminated and/or has accumulated mutations.
http://hg.wustl.edu/hdk_lab_manual/plasmid/plsmid0...
Added: Tue May 14 2002, Hits: 2912, Reviews: 0
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