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Protocols
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Antigen Design & Sera Purification
(Genosys)
This protocol describes peptide selection and design, coupling strategy, and carrier proteins which are important factors in anti-peptide antisera generation.
http://www.genosys.co.uk/custom_antisera/tech_info...
Added: Tue May 14 2002, Hits: 361, Reviews: 0
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Preparation Of Peptide-KLH Conjugates For Immunization
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/ab1.htm...
Added: Tue May 14 2002, Hits: 936, Reviews: 0
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Protocol for Heat-induced Epitope Retrieval (HIER)
(R&D Systems, Inc.)
A detailed protocol for HEIR is accompanied by a printable schematic
http://www.rndsystems.com/ihc_detail_objectname_he...
Added: Tue Jun 01 2010, Hits: 1258, Reviews: 0
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Testing Rabbit Bleeds By Elisa
(Mitchison Lab)
You can test whether or not you have gotten an immune response to the peptide and how strong that immune response is by doing ELISAs against peptide conjugated to BSA.
http://mitchison.med.harvard.edu/protocols/ab2.htm...
Added: Tue May 14 2002, Hits: 892, Reviews: 0
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