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Protocols
- Monoclonal Antibody Production (Janos Luka Ph.D., Department of Pathology, Eastern Virginia Medical School)
Detailed guide to monoclonal antibody production from mice immunization to fusion of spleen cells and myeloma cells to screening of antibody.
http://herpesvirus.tripod.com/research/HYBRIDOM.ht...
Added: Fri Apr 16 2004, Hits: 8431, Reviews: 0 Write review Cached - Production of monoclonal antibody by hybridoma fusion (iProtocol)
A simple and contemporary protocol for the development of monoclonal antibodies using hybridoma fusion in immune mice.
http://iprotocol.mit.edu/protocol/335.htm
Added: Fri Oct 17 2003, Hits: 664, Reviews: 0 Write review Cached - Production of Monoclonal Antibody (Chisholm Lab, Northwestern University Medical School)
Covers every step of making monoclonal antibodies starting from supplies, immunization, fusion, prepare immunized spleen cells, screening, affinity purification of IgG using G-beads and more...
http://dicty.cmb.nwu.edu/Chis_lab/Lab%20Manual/imm...
Added: Tue May 14 2002, Hits: 1199, Reviews: 0 Write review Cached -
Expanding Hybridoma Clones
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 313, Reviews: 0 Write review Cached - Fusion and Cloning (Contributed by Nanci Donacki)
Procedure for establishing hybridoma in one step
Added: Tue May 14 2002, Reviews: 0 Write review -
Monoclonal Antibody Production
(Kitto Lab, The University of Texas at Austin)
Describes procedures including mouse immunization, hybridoma fusion, screening and clone expansion.
http://www.cm.utexas.edu/kitto/Kittolabpage/Protoc...
Added: Mon Apr 19 2004, Hits: 190, Reviews: 0 Write review Cached -
Monoclonal Antibody Screening by Direct ELISA
(Bjorkman Group, Howard Hughes Medical Institute at California Institute of Technology)
http://www.its.caltech.edu/%7Ebjorker/ELISA_.pdf
Added: Wed Aug 14 2002, Hits: 2734, Reviews: 0 Write review -
Preparation of Monoclonal Antibody
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
Protocol for making hybridoma and antibody
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 561, Reviews: 0 Write review Cached -
Tips and hints for the storage of antibodies
(Synaptic Systems)
http://www.sysy.com/faqs/storage.html
Added: Tue May 14 2002, Hits: 345, Reviews: 0 Write review Cached