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Protocols
- Microinjection of Xenopus Oocyte (Gene Delivery Group, University of Birmingham)
This protocol covers: Preparation of the oocytes, Selection of oocytes for microinjection, Sample preparation, Microinjection of the oocytes, Assaying for gene expression.
http://web.bham.ac.uk/can4psd4/brum/micro.html
Added: Mon Sep 09 2002, Hits: 278, Reviews: 0
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1-cell embryo transfer into pseudopregnant recipient female mouse
(Gary A. J. Brown)
Detailed protocol with illustration
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 254, Reviews: 0
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Aggregation of ES Cells and Eight Cell Stage Embryos
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 88, Reviews: 0
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Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Beta-gal Solution Assay : for quantitative analysis
(Mike A. Dyer)
http://axon.med.harvard.edu/~cepko/protocol/mike/B...
Added: Tue May 14 2002, Hits: 136, Reviews: 0
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Beta-Gal Staining of the Murine Embryo
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Blastocyst Transfer
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Collection of Morulae and Earlier
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 72, Reviews: 0
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Embryo Manipulation Pipettes (Flushers etc.)
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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ES Cell Pipette Manufacture
(Gary A. J. Brown)
Make injection needles yourself
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 155, Reviews: 0
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Flushing Out of Blastocysts
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 81, Reviews: 0
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Harvesting of Cells for Blastocyst Injection
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 75, Reviews: 0
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Intracytoplasmic Spermatocyte Injection (ICSI)
(Brinkmann)
Detailed technique guide for microinjection.
http://www.brinkmann.com/ECET_appl4.asp
Added: Tue May 14 2002, Hits: 64, Reviews: 0
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Micropipette Beveling
(Gary A. J. Brown)
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 154, Reviews: 0
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PCR Genotyping from the Embryo Yoke Sac
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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PCR Genotyping of Embryos and Blastocysts
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 72, Reviews: 0
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Preparation of Microinjection Pipettes
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Sample Preparation for Microinjection
(Brinkmann)
Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm [1]. This capillary is back loaded with the substance to be transferred into the cells cultured for microinjection. Typical substances include purified antibodies, DNA, RNA, peptides, or oligonucleotides.
http://www.brinkmann.com/ECET_appl2.asp
Added: Tue May 14 2002, Hits: 79, Reviews: 0
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Sending Sera to Testing for Mouse Pathogens
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 41, Reviews: 0
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Superovulation of Mice
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 70, Reviews: 0
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Vasectomy of Male Mice
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 66, Reviews: 0
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Vasectomy of Mouse
(Gary A. J. Brown)
Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at 6-8 weeks of age.
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 151, Reviews: 0
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Whole Mount X-gal Histochemistry of Transgenic Animal Tissues and Cultured Cells
(Eric Mercer)
Provides detailed procedures.
http://www.muridae.com/wmc/docs/lacZ_bible.html
Added: Tue May 14 2002, Hits: 519, Reviews: 0
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