Protocols
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AFLP
(Mannie Liscum and Paul Oeller, Department of Plant Biology, Stanford)
AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields, including plant and animal breeding, medical diagnostics, forensic analysis and microbial typing, to name a few.
http://www-ciwdpb.stanford.edu/publications/method...
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DNA Amplification Fingerprinting Protocol
(C. S. Prakash)
DNA is amplified by PCR. The resulting PCR products are separated on polyacrylamide gel and silver stained for DNA visualization.
http://agriculture.tusk.edu/biotech/dnafngr.html
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DNA Fingerprinting (Agarose Gel)
(Caltech)
http://www.tree.caltech.edu/protocols/agfp.html
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DNA Fingerprinting (Polyarylamide Gel
( )
) (Caltech)
BAC DNA sample and gel preparation, gel running and more
http://www.tree.caltech.edu/protocols/fingerprinti...
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High Resolution Genetic Footprinting
(Stanford)
Genetic footprinting is a technique for high-resolution mapping of the functional organization of a cloned gene. An in vitro transposition reaction with purified retroviral integrase is used to generate a large library of mutants, each of which bears an insertion or block substitution mutation of defined sequence at some position within the gene. The library of mutants is simultaneously subjected to one or more genetic selections, and DNA is made from the library of mutants before and after selection. The presence or absence of individual mutants within each population is determined by PCR analysis, in which each mutant within the library gives rise to a PCR product of unique electrophoretic mobility. Comparison of PCR product bands before and after selection allows reconstruction of the features of the gene that are essential for the function that has been selected.
http://cmgm.stanford.edu/pbrown/gfpage.html
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RLCS protocol for plant mRNA
(Makoto Kawase)
Detailed Restriction Landmark cDNA Scanning (RLCS) protocol.
http://www.geocities.jp/millet_kawase/rlcs/RLCS_pr...
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RLGS protocol for plant genome
(Makoto Kawase)
Detailed Restriction Landmark Genomic Scanning (RLGS) protocol. Although this protocol is for plant genome, it can also be applied to other genome.
http://www.geocities.jp/millet_kawase/RLGS/protoco...
Added: Tue May 14 2002, Hits: 845, Reviews: 0 Write review