Protocols
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Chromosome Staining
(Britt Lab, UC Davis)
Stain arabidopsis meiotic chromosomes from pollen meiocytes
http://www-plb.ucdavis.edu/labs/britt/chromosomest...
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Conventional Giemsa Stain
(Primate Cytogenetics Network)
Conventional staining techniques are used to uniformly stain chromosomes and leave the centromeres constricted, thus enabling the measurement of chromosome length, centromeric position, and arm ratio.
http://homepage.mac.com/wildlifeweb/cyto/text/Giem...
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Conventional Giemsa Stain 2
(Primate Cytogenetics Network)
Conventional staining techniques are used to uniformly stain chromosomes and leave the centromeres constricted, thus enabling the measurement of chromosome length, centromeric position, and arm ratio.
http://homepage.mac.com/wildlifeweb/cyto/text/Giem...
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DAPI/Distamycin A Staining
(Primate Cytogenetics Network)
The DAPI/Distamycin A staining technique is useful in identifying pericentromeric breakpoints in chromosomal rearrangements and in identifying chromosomes that are too small for standard banding techniques. Also, DAPI/DA is the method of choice for Yqh chromsome material in suspected Y autosome translocations.
http://homepage.mac.com/wildlifeweb/cyto/text/DAPI...
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Leishman's Stain
(Primate Cytogenetics Network)
http://homepage.mac.com/wildlifeweb/cyto/text/Leis...
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NOR-staining (Silver Nucleolar Organizing Region Staining)
(Primate Cytogenetics Network)
Chromosomes are treated with silver nitrate solution which binds to the Nucleolar Organizing Regions (NOR), i.e., the secondary constrictions (stalks) of acrocentric chromosomes.
http://homepage.mac.com/wildlifeweb/cyto/text/NOR-...
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Vital Staining of Chromosomes with Hoechst 33258
(Yu-li Wang's Lab, University of Massachusetts Medical School )
http://ylwang.umassmed.edu/protocol/cfs/hoechst.ht...
Added: Fri Oct 17 2003, Hits: 1037, Reviews: 0 Write review Cached