Protocols
- G-Banding (GTL) (Primate Cytogenetics Network)
Chromosomes are G-banded to facilitate the identification of structural abnormalities. Slides are dehydrated, treated with the enzyme trypsin, and then stained.
http://homepage.mac.com/wildlifeweb/cyto/text/G-ba...
Added: Fri Apr 02 2010, Hits: 1720, Reviews: 0 Write review Cached - Q-Banding (Primate Cytogenetics Network)
Chromosomes are treated with quinicrine mustard solution, a fluorescent stain, to identify specific chromosomes and structural rearrangements. It is expecially useful for distinguishing the Y chromosome (also Y bodies in interphase nuclei) and various polymorphisms involving satellites and centromeres of specific chromosomes.
http://homepage.mac.com/wildlifeweb/cyto/text/Q-ba...
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C-Banding
(Primate Cytogenetics Network)
To specifically stain the centromeric regions and other regions containing constitutive heterochromatin, i.e., the secondary contrictions of human chromsomes 1, 9, 16, and the distal segment of the Y chromosome long arm.
http://homepage.mac.com/wildlifeweb/cyto/text/C-ba...
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G-banding for analysing mouse chromosomal rearrangements
(Practical Approach Online, Oxford University Press)
http://www.oup.co.uk/pdf/pas/13-6a-2.pdf
Added: Wed Sep 04 2002, Hits: 1085, Reviews: 0 Write review -
Giemsa Reverse Banding (RHG) or R Banding
(Primate Cytogenetics Network)
R-banding methods are useful for analyzing deletions or translocations that involve the telomeres of chromosomes.
http://homepage.mac.com/wildlifeweb/cyto/text/%28R...
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R Banding by Fluorescence Using Acridine Orange (AO)
(Primate Cytogenetics Network)
R-banding methods are useful for analyzing deletions or translocations that involve the telomeres of chromosomes.
http://homepage.mac.com/wildlifeweb/cyto/text/%28A...
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Replication Banding
(Waisman Center Cytogenetics Lab, University of Wisconsin - Madison)
Provides differential staining of early and late replicating areas of the chromosomes. Allows identification of the early replicating X chromosome. Can be used for marker chromosome analysis. When Bromo deoxyuridine is added at the end of culture late replicating areas will stain pale and early replicating areas will stain dark.
http://www.slh.wisc.edu/cytogenetics/Protocols/Sta...
Added: Mon Dec 09 2002, Hits: 59, Reviews: 0 Write review Cached -
T-Banding
(Primate Cytogenetics Network)
T-banding is used to stain the telomeric regions of chromosomes for cytogenetic analysis.
http://homepage.mac.com/wildlifeweb/cyto/text/T-ba...
Added: Fri Apr 02 2010, Hits: 524, Reviews: 0 Write review Cached