Protocols
- Chromosome Conformation Capture (3C) (Epigenome NoE)
The 3C (Chromosome Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al., 2002). Cells are fixed with formaldehyde which forms DNA-protein and protein-protein cross-links between regions of the genome in proximity (see figure 1). Subsequent restriction enzyme digestion and intra-molecular ligation produces novel junctions between restriction fragments in proximity in the nucleus. Novel ligation products can be detected by PCR.
http://www.epigenome-noe.net/WWW/researchtools/pro...
Added: Wed Mar 23 2011, Hits: 963, Reviews: 0 Write review -
Chromosome conformation capture
(Abcam)
http://www.abcam.com/index.html?pageconfig=resourc...
Added: Wed Mar 23 2011, Hits: 633, Reviews: 0 Write review -
Chromosome Conformation Capture (3C) Assay
(Epigenome NoE)
The 3C (Chromosome Conformation Capture) technique generates a population average measurement of juxtaposition frequency between any two genomic loci, thus providing information on their relative proximity in the nucleus (Dekker et al., 2002). Cells are fixed with formaldehyde which forms DNA-protein and protein-protein cross-links between regions of the genome in proximity (see figure 1). Subsequent restriction enzyme digestion and intra-molecular ligation produces novel junctions between restriction fragments in proximity in the nucleus. Novel ligation products can be detected by PCR.
http://www.epigenome-noe.net/WWW/researchtools/pro...
Added: Wed Mar 23 2011, Hits: 780, Reviews: 0 Write review