Top : General Laboratory Techniques : Water Treatment with DEPC

Summary: Diethyl Pyrocarbonate (DEPC or DEP) is effective as a nuclease inhibitor. Typically a 0.1% solution is used to inactive RNase.
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  • DEPC Water Treatment (OpenWetWare)
    Diethyl pyrocarbonate (DEPC) is an efficient, nonspecific inhibitor of RNases. It is typically used to treat water and solutions before working with easily degraded RNA. DEPC reacts with amine, hydroxy and thiol groups of proteins thereby inactivating RNAses (and other enzymes).
    http://openwetware.org/wiki/DEPC
    Added: Tue Feb 17 2009, Hits: 8304, Reviews: 0  Write review   Cached
  • DEPC PDF file (Sigma)
    Before you know anything about DEPC treatment of water, you must read this informative product sheet of DEPC.
    http://www.sigmaaldrich.com/etc/medialib/docs/Sigm...
    Added: Tue Feb 17 2009, Hits: 5973, Reviews: 0  Write review  
  • RNase and DEPC Treatment (Ambion)
    Some basic facts about RNase and DEPC treatment.
    http://www.ambion.com/techlib/tb/tb_178.html
    Added: Tue Feb 17 2009, Hits: 3939, Reviews: 0  Write review   Cached
  • Water Treatment with DEPC (Protocol Online)
    Treat water with DEPC to eliminate RNase
    Added: Thu May 08 2003, Reviews: 0  Write review  
  • Water Treatment with DEPC PDF file (Sigma-Aldrich)
    Protocol to treat water with DEPC
    http://www.sigmaaldrich.com/etc/medialib/docs/Sigm...
    Added: Tue Jun 23 2009, Hits: 4083, Reviews: 0  Write review  
  • Water Treatment with DEPC and other material treatment PDF file (MolGen)
    http://molgen.biol.rug.nl/molgen/files/RNA_materia...
    Added: Tue May 14 2002, Hits: 2976, Reviews: 0  Write review