Protocols
- Cell counting with an hemacytometer (Giorgio Cattoretti, Columbia University)
Detail protocol on how to use hemacytometer to count cells.
http://icg.cpmc.columbia.edu/cattoretti/Protocol/F...
Added: Sun Jul 28 2002, Hits: 19821, Reviews: 0 Write review Cached - Cell Counting/ Live-Dead Discrimination using EB and Acridine Orange (Flow Cytometry Core Facility, University of Chicago)
This is a microscopy based application for definitive discrimination of live and dead cells. Trypan Blue exclusion notoriously over estimates the number of dead cells. Using this protocol, you can obtain a more accurate count of cells.
http://iacf.uchicago.edu/Protocols/CellCounting.ht...
Added: Sat Feb 21 2009, Hits: 1752, Reviews: 0 Write review Cached - Counting and Determining the Viability of Cultured Cells
(Journal of Visualized Experiments)
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A hemacytometer is a thick glass slide with a central area designed as a counting chamber. Cell suspension is applied to a defined area and counted so cell density can be calculated.
http://www.jove.com/index/details.stp?ID=752
Added: Wed Feb 11 2009, Hits: 3633, Reviews: 0 Write review Cached - Trypan Blue Viability Test (Dr. Volker Briese, Universitäts-Frauenklinik Rostock)
Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
http://www-ufk.med.uni-rostock.de/lablinks/protoco...
Added: Mon Oct 28 2002, Hits: 6494, Reviews: 0 Write review Cached - Use of the Hemacytometer for the Determination of Cell Numbers (Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas)
Counting cells by the use of a hemacytometer is a convenient method. The hemacytometer consists of two chambers, each of which is divided into nine 1.0 mm squares...
http://www.genetics.emory.edu/docs/Caspary%20Proto...
Added: Sun Jul 28 2002, Hits: 11790, Reviews: 0 Write review Cached -
Cell Counting
(Sefton Lab, Salk Institute)
http://pingu.salk.edu/~sefton/Hyper_protocols/coul...
Added: Tue May 14 2002, Hits: 7691, Reviews: 0 Write review Cached - Cell Counting with the Sedgewick-Rafter Chamber and Whipple Micrometer Disc (Protocol Online)
A step by step protocol to standardize the counting of cells using the Sedgewick-Rafter Chamber and Whipple Micrometer Disc. Statistically significant counts of protists in cultures of millions/ml may be obtained by directly counting less than 0.1% .
Added: Tue Mar 08 2011, Reviews: 0 Write review -
Cell Counts Using a Hemocytometer
(Donis-Keller lab)
The purpose of this procedure is to determine the cell density of the culture. Cell cultures always have some dead cells; the viable and non-viable cells can be distinguished with the use of trypan blue dye and a hemacytometer. Living cells will not take up the dye while dead cells do.
http://hg.wustl.edu/hdk_lab_manual/hcc/hcc7.html
Added: Tue May 14 2002, Hits: 7259, Reviews: 0 Write review Cached -
Eukaryote Growth Dynamics
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
For determining cell doubling time.
http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
Added: Tue May 14 2002, Hits: 1434, Reviews: 0 Write review Cached -
Hemacytometer Reference Guide
(BD PharMingen)
Illustrated instruction for using a hemacytometer to count cells
http://www.bdbiosciences.com/discovery_labware/tec...
Added: Tue May 14 2002, Hits: 4371, Reviews: 0 Write review Cached -
Platelet Counting
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain (UCL))
how to count platelets in blood sample.
http://www.icp.ucl.ac.be/mexp/vipg_plcou.html
Added: Mon Dec 09 2002, Hits: 1571, Reviews: 0 Write review Cached -
Use of a Hemacytometer
(P.J. Hansen Lab)
Very detailed guide on counting cells using a hemacytometer
http://www.animal.ufl.edu/hansen/protocols/hemacyt...
Added: Tue May 14 2002, Hits: 5659, Reviews: 0 Write review Cached -
Viability Cell Count
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
Added: Tue May 14 2002, Hits: 4601, Reviews: 0 Write review Cached