Protocols
- Cell-Free System for the Examination of Apoptotic Activity (CellDeath.de)
Activation of apoptotic activity in cytoplasmic extracts and measurement of the resulting caspase enzymatic acticity,br>Activation of apoptotic activity in cytoplasmic extracts western blot analysis Reconstitution of activated cytoplasmic extracts with isolated nuclei. Analysis of apoptotic activity by qualitative DNA laddering assay and DAPI staining Reconstitution of activated cytoplasmic extracts with isolated radioactive nuclei. Analysis of apoptotic activity by quantitative, radioactive DNA fragmentation assays or DAPI staining.
http://www.celldeath.de/apometh/cfsystem.html
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Caspase Enzymatic Assay
(CellDeath.de)
Caspase enzymatic activity is measured following incubation of cytoplasmic extracts in the presence or absence of activating stimuli
http://www.celldeath.de/apometh/cfsystem.html#casp...
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Isolation of Cell Nuclei Extracts for Cell-Free Apoptotic Assay
(CellDeath.de)
http://www.celldeath.de/apometh/nucprep.html
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Preparation of Cytoplasmic Extracts for Cell-Free Apoptotic Assay
(CellDeath.de)
Cells are grown to 80% confluency, then harvested, washed and disrupted in KPM buffer by freezing-thawing cycles with liquid nitrogen.
http://www.celldeath.de/apometh/cytoextr.html
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