Protocols
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AA & Metabolite Quantitation of Cell Pellets Post-AA Labeling
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0024.h...
Added: Thu Jul 25 2002, Hits: 1835, Reviews: 0 Write review Cached -
AA & Metabolite Quantitation of Media Post-AA Labeling
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0025.h...
Added: Thu Jul 25 2002, Hits: 1669, Reviews: 0 Write review Cached -
Arachidonic Acid Labeling
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0023.h...
Added: Thu Jul 25 2002, Hits: 1649, Reviews: 0 Write review Cached -
Column Purification of Demethylated Sphingomyelin
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0031.h...
Added: Thu Jul 25 2002, Hits: 1583, Reviews: 0 Write review Cached -
Desalting Acidic Glycosphingolipids
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0022.h...
Added: Thu Jul 25 2002, Hits: 1514, Reviews: 0 Write review Cached -
DGK Assay
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0029.h...
Added: Thu Jul 25 2002, Hits: 1583, Reviews: 0 Write review Cached -
DGK Membrane Preparation
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0030.h...
Added: Thu Jul 25 2002, Hits: 1645, Reviews: 0 Write review Cached - Estimation of Fat in Milk Samples (Protocol Online)
This protocol is a simple and explained with brief introduction and principle. This is highly useful for the researchers working in the field of dairy.
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Extracting cholesterol and lipids from cells
(The Molecular Probes Technology Network)
This a general method that may not work for all cell/tissue samples - tweak the solvent volumes per quantity of cells, centrifugation time and sonication may be applied for samples that are difficult to disrupt. For chloroform - handle under a chemical safety hood.
http://molecularprobestechnologynetwork.community....
Added: Fri Oct 15 2010, Hits: 1733, Reviews: 0 Write review -
Glycosphingolipid analysis
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0034.h...
Added: Thu Jul 25 2002, Hits: 1845, Reviews: 0 Write review Cached -
In vitro Sphingomyelinase Assay
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0035.h...
Added: Thu Jul 25 2002, Hits: 1568, Reviews: 0 Write review Cached -
Isolation of LDL(Low Density Lipoprotein) From Human Plasma
(John Garland, The Biochemistry and Molecular Biology Department of The James H. Quillen College of Medicine in Johnson City, TN)
http://qcom.etsu.edu/biochem/protocols/ldl.htm
Added: Sat Sep 14 2002, Hits: 185, Reviews: 0 Write review Cached -
Isolation of Llipoprotein
(Dr. Norbert H. Haunerland, Simon Fraser University)
Isolation of a lipoprotein from insect blood by density gradient centrifugation and analysis of its apoproteins by electrophoresis.
http://www.sfu.ca/bisc/bisc-429/lipoprotein1.html
Added: Sun Sep 22 2002, Hits: 2149, Reviews: 0 Write review Cached -
Labelled Sphingmyelin Synthesis
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0036.h...
Added: Thu Jul 25 2002, Hits: 1441, Reviews: 0 Write review Cached -
Lipid extraction
(Ivan J Delgado)
http://ivaan.com/protocols/206.html
Added: Sun Mar 01 2009, Hits: 3001, Reviews: 0 Write review Cached -
Lipid Transmethylation
(Dr. Norbert H. Haunerland, Simon Fraser University)
Transmethylation of lipids and extraction of methyl derivatives
http://www.sfu.ca/bisc/bisc-429/transmethylation.h...
Added: Sun Sep 22 2002, Hits: 1713, Reviews: 0 Write review Cached -
Liposome Preparation
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
Method for incorporating proteins into liposomes for liposome swelling assay or as an alternative antigen presentation method.
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 1189, Reviews: 0 Write review Cached -
Liposome Swelling Assay
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 777, Reviews: 0 Write review Cached -
Methylation of Fatty Acids (Kropinski Method)
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
To methylate fatty acids in whole cells or lipopolysaccharide
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 746, Reviews: 0 Write review Cached -
Neutralizing Arachidonic Acid
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0026.h...
Added: Thu Jul 25 2002, Hits: 1358, Reviews: 0 Write review Cached -
Phosphate Assay
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0004.h...
Added: Thu Jul 25 2002, Hits: 1740, Reviews: 0 Write review Cached -
Phosphate Assay by Suprya Jayadev
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0038.h...
Added: Thu Jul 25 2002, Hits: 1641, Reviews: 0 Write review Cached -
Preparation of bOG-DOPG mixed micelles
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0027.h...
Added: Thu Jul 25 2002, Hits: 1366, Reviews: 0 Write review Cached -
Purification of Demethylated Sphingomyelin
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0039.h...
Added: Thu Jul 25 2002, Hits: 1391, Reviews: 0 Write review Cached -
Purification of Demethylated Sphingomyelin
(Hannun/Obeid Lab of Medical University of South Carolina)
http://www.musc.edu/bcmb/ceramide/protocols/0032.h...
Added: Thu Jul 25 2002, Hits: 1367, Reviews: 0 Write review Cached