Protocols
- Two dimensional peptide mapping (Sefton Lab, Salk Institute)
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years
http://pingu.salk.edu/~sefton/Hyper_protocols/Tryp...
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CNBr mapping
(Sefton Lab, Salk Institute)
Kunxin Luo/James Hardwick CNBr Cleavage Procedure
http://pingu.salk.edu/~sefton/Hyper_protocols/CNBR...
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Peptide map prediction
(Sefton Lab, Salk Institute)
The programs Mobility and Pepsort in GCG package can be used together to predict and plot the theoretical mobilities of peptides in two dimensional thin layer analysis. The plots show electrophoresis in the first dimension and ascending chromatography in the second dimension
http://pingu.salk.edu/~sefton/Hyper_protocols/peps...
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Peptide Mapping Analysis Techniques
(R. Aebersold, Vrije Universiteit in Amsterdam)
In-gel digestion procedure for peptide mass mapping of picomole to subpicomole quantities of protein derived from coomassie- or silver stained polyacrylamide gels. First, SDS is removed from the gel prior to digestion, in several ammoniumcarbonate/acetonitrile washing steps. The excised gel pieces are subsequently dried and rehydrated with enzyme in buffer.
http://www.bio.vu.nl/vakgroepen/mnb/proteomics/6.i...
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V8 partial proteolytic mapping
(Sefton Lab, Salk Institute)
Partial proteolysis with Staph. aureus V8 protease
http://pingu.salk.edu/~sefton/Hyper_protocols/Clev...
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