Ct variation in the internal control - (Oct/03/2005 )
Hi!
I want to know which level of difference is acceptable for an internal control between the control sample and the treated sample. I use GAPDH and, for instance, in the control sample the Ct is 20 and in the treated one is 20.2, which I think is correct. But in other cases, the Ct is 20 in the control and 21 or 22 in the treated. Which is the limit of difference I can accept? 0.5 cycles? 1 cycle?
Thank you very much!
Neus
-Neus-
QUOTE (Neus @ Oct 3 2005, 01:13 PM)
Hi!
I want to know which level of difference is acceptable for an internal control between the control sample and the treated sample. I use GAPDH and, for instance, in the control sample the Ct is 20 and in the treated one is 20.2, which I think is correct. But in other cases, the Ct is 20 in the control and 21 or 22 in the treated. Which is the limit of difference I can accept? 0.5 cycles? 1 cycle?
Thank you very much!
Neus
I want to know which level of difference is acceptable for an internal control between the control sample and the treated sample. I use GAPDH and, for instance, in the control sample the Ct is 20 and in the treated one is 20.2, which I think is correct. But in other cases, the Ct is 20 in the control and 21 or 22 in the treated. Which is the limit of difference I can accept? 0.5 cycles? 1 cycle?
Thank you very much!
Neus
Hello, I have the same question actually. Are there any articles on this issue?
-StaticApnea-
QUOTE (Neus @ Oct 3 2005, 04:13 AM)
Hi!
I want to know which level of difference is acceptable for an internal control between the control sample and the treated sample. I use GAPDH and, for instance, in the control sample the Ct is 20 and in the treated one is 20.2, which I think is correct. But in other cases, the Ct is 20 in the control and 21 or 22 in the treated. Which is the limit of difference I can accept? 0.5 cycles? 1 cycle?
Thank you very much!
Neus
I want to know which level of difference is acceptable for an internal control between the control sample and the treated sample. I use GAPDH and, for instance, in the control sample the Ct is 20 and in the treated one is 20.2, which I think is correct. But in other cases, the Ct is 20 in the control and 21 or 22 in the treated. Which is the limit of difference I can accept? 0.5 cycles? 1 cycle?
Thank you very much!
Neus
Hi Neus,
maybe you should use a different internal control, like 28s rRNA. Actin and metabolic enzymes are always critical, although everywhere published, since they become regulated easily.
Cheers,
ophris
-ophris-