DNA extraction from soil - (Oct/01/2005 )
I am interested in extracting DNA from soil for metagenomic studies.so the objective is to get maximum amount of DNA. I have used the MoBio kit and also extracted DNA with Zhou's method...I would be very thankful if some one could guide me on a method in which there is maximum DNA yield from soil.
-jsn-
QUOTE (jsn @ Oct 1 2005, 05:25 AM)
I am interested in extracting DNA from soil for metagenomic studies.so the objective is to get maximum amount of DNA. I have used the MoBio kit and also extracted DNA with Zhou's method...I would be very thankful if some one could guide me on a method in which there is maximum DNA yield from soil.
Hi,
Just curious to know if Mbio kit worked.
I tried Freeze-thaw,proteinase K and phenol-chloroform method extraction method which worked ok.Let me know if you tried any new method and were successful.
envt 006
-envt 006-
QUOTE (envt 006 @ Jul 3 2006, 08:09 AM)
Hi,
Just curious to know if Mbio kit worked.
I tried Freeze-thaw,proteinase K and phenol-chloroform method extraction method which worked ok.Let me know if you tried any new method and were successful.
envt 006
Just curious to know if Mbio kit worked.
I tried Freeze-thaw,proteinase K and phenol-chloroform method extraction method which worked ok.Let me know if you tried any new method and were successful.
envt 006
Hi, I wonder, by using that metods, was the yield high? I have used Dong et al 2006, but I have low yield also. Thanks
-rif_researcher-