Getting crazy because of cell contamination - (Sep/29/2005 )
Hi,
I yesterday plated out a small experiment (2 48-well plates) using the medium I made on Monday. These medium still looks good today, so maybe it was though the warmwaterbath causing the contamination...
If however the contamination again is present after the weekend, I will seriously clean the CO2-incubator following your guidelines, Pesji! Thanks a lot for the PDF, finally a paper which gives some very practical tips, in stead of the general and superficial tips I found elsewhere! 
Augmenting the temperature in the incubator as high as possible seems maybe a very efficient method to me, but unfortunately our incubator is already quite old and I don't want to risk a defect... New incubators have been ordered some months ago and I anyway hope they will be delivered as soon as possible!
By the way: we now have 1 big incubator consisting of 2 distinct units which can be opened apart from each other. But the contaminations occur in both units.
But of course, I hope that, after this week without contaminations, a cleaning of the incubator is just no more needed. Maybe my nightmare just finally disappeared... let's hope!! I'll certainly let you know on Monday.
An xx
Well Anneke I wish you as much luck as my fingers crossed (including my toes;o)!
pesji
The story continues...
This week, I didn't have any contamination. However, some of my colleagues had, so the problem still is there.
Yesterday, our new incubators have been delivered (finally! :-)). We have sterilized them by increasing the temperature before using them and we are now all hoping that the contamination now completely belongs to the past!!
Greetz, An
This week, I didn't have any contamination. However, some of my colleagues had, so the problem still is there.
Yesterday, our new incubators have been delivered (finally! :-)). We have sterilized them by increasing the temperature before using them and we are now all hoping that the contamination now completely belongs to the past!!
Greetz, An
I will keep my fingers crossed for you !
Pesji
Many labs do not use antibiotics as it can give rise to resistant strains which are pesky to get rid of. In addition, if you are using these cells for animal experiments you do not want to be using antibiotics.
With good cell culture technique antibiotics should not be needed except for break-outs. That being said, perhaps this grad student should consider to use antibiotics in her lab until her infections clear up.
Also, Anneke, you MUST TEST FOR MYCOPLASMA IMMEDIATELY!!
Is there any reason you don't use antibiotics? If you try adding 10ml per 500ml and you are still getting apparent contamination then it can't be a bacterial problem!
Hello,
We have brought a contaminated cell line and contaminated medium to the microbiology group of our university and it turned out to be Staphylococcus.
So now, our search of the possible source of contamination will hopefully lead to a solution.
An
We have brought a contaminated cell line and contaminated medium to the microbiology group of our university and it turned out to be Staphylococcus.
So now, our search of the possible source of contamination will hopefully lead to a solution.
An
Well Anneke that's quite a progress even if I don't get how you can get such a persistent contamination it's a bit frightening. Just Hope that you will solve that annoying troubel is the new incubator safe from this or did you got it also contaminated ?
Pesji
