compatible host for pUC19 - (Sep/06/2005 )
hi
im trying to clone 1.2kb pcr product and find it not easy at all. i've being using pUC19 and xlblue MRF which were available in our lab but came to know jm109 is the compatible strain for pUC19.i would be grateful if any body can tell me where i can order these two. i would like to get them from the same company. im not sure if it would affect the performance if i use products from different companies, 'cos the discriptions i find in product sheets of different companies differ.
thanks in advance
chandima
-chandima-
hi
jm109 competent cells can be purchased from NEB (here)
fred
-fred_33-
hi fred_33
thanks
cheer chandima
-chandima-