Is polymerase slippage related to low fidelity? - DNA polymerase slippage during PCR (Sep/03/2005 )

Hi all,

Here's a question that has been bothering me for a while...

Stutter peaks (in fragment analysis - Genescan) in PCR targeting dinucleotide repeats is caused by DNA polymerase slippage mispairing.

By using proofreading enzymes with high fidelity, will that solve the problem of slippage?

Enzyme slippage during PCR is not equivalent to misincorporation of nucleotides right?

Waiting to be enlighten with thanks...

Sherry

PCR slippage is actually linked to low polymerase processivity, although low fidelity will contribute somewhat to the problem. If you think about it this makes sense because in slippage the 3' end of the growing strand is perfectly paired with a looped out region.

Daniel

DNA sequencing

Thanks Daniel!