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prepare loading dye - (Aug/25/2005 )

hi. just want to ask recipe for preparing gel loading dye. smile.gif cost cutting... we have bromophenol, edta and tris and otehrs ... thats why i just want to prepare it on my own insted of buying. I just want to ask how much of each? in terms of volume. How many ml of this, how many ml of that. thanks

-rina77-

Be more specific please.

Loading dye for protein or dna.

Agarose gels or acrylamide.

Denaturing or non-denaturing gels.

Consult Sambrook. If you don't have them, answer the above and I can supply the answers.

-pBluescript-