INTERNAL CONTROLS IN MICROARRAY - (Aug/04/2005 )
WHY DO WE NEED INTERNAL CONTROLS IN MICROARRAY? Is this for normalization?
I wonder what should i do with the internal controls. Should i label the internal controls and then add into my samples or what should i do? Any ideas? I will work with long 35k human oligonucleotide array.
I hope for your answers!
Thank you!
-mioarray-
QUOTE (mioarray @ Aug 5 2005, 12:58 AM)
WHY DO WE NEED INTERNAL CONTROLS IN MICROARRAY? Is this for normalization?
I wonder what should i do with the internal controls. Should i label the internal controls and then add into my samples or what should i do? Any ideas? I will work with long 35k human oligonucleotide array.
I hope for your answers!
Thank you!
I wonder what should i do with the internal controls. Should i label the internal controls and then add into my samples or what should i do? Any ideas? I will work with long 35k human oligonucleotide array.
I hope for your answers!
Thank you!
I am not an expert, but I would think that you print a probe robustly hybridize to a certain transcript that you have. then spike the transcript with a know concentration in you sample, this way the signal of the probe you printed can be used as an internal controal.
-cyberpostdoc-
Have you heard about using external controls in microarry or are there only internal controls?
-mioarray-