RAPD PCR help - (Aug/04/2005 )

Hi,

I know this is a pretty basic question but I need to know the answer. Can you tell me if you need to use forward and reverse primer during random amplification polymorphic DNA (RAPD)?

THanks

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No. But you might find that you get more reproducible results if you do - that is my experience anyway

Daniel

DNA sequencing software

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No, RAPD normally uses only one primer, a random 10-mer which function as both forward and reverse primer. This is possible because a 10-mer sequence by chance occur relatively frequently, and thus the primer binds at random on both DNA strands.

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