Problems with MES - autoclavable?? (Aug/04/2005 )
Hi
Can I to esterilize a 10% solution of MES (2-Morpholinoethanesulfonic acid, monohydrate) by autoclave?
Thanks
Jobsky
hi
yes you can
but MOPS doesn't support DEPC... (i don't know if you mind but i had to thow away a fresh solution because my boss told me that after...)
MES is not the same as MOPS by the way
I`m confused
MOPS is autoclavable but MES no? both?
help?
thank you
jobsky
hi
sorry for late reply.
just ask the manufacturer. I can't tell more cause i've made a mistake in products. But may i ask you to tell us the information?
Thanks.
Fred
Hi fred33
There is not answer from sigma yet ...
But i´ll share the information of course.
I´m waiting.
sigma answer...
We do not
recommend that MES buffer solutions be sterilized by autoclaving, as the
solutions turn yellow and degradation may occur. MES buffer solutions
should be sterilized by sterile filtration through a 0.22 micron filter.
jobsky