RNA isolation - question about kits (Aug/04/2005 )
Dear All,
Does anyone can recommand a kit for microbial RNA isolation. I am an absolute greenhorn in terms of working with RNA... I need the RNA for RT-PCR.
I thought the Ambion kit might be good. But then I saw that one is supposed to by such a vortex adapter as well (for a 10min horizontal vortexing step). Our vortexer are ancient and I don't believe any adapter would fit onto it. Does anyone know if there is any way to use the Ambion kit even without buying that adapter?
Best,
Freiberger
-Freiberger-
QUOTE (Freiberger @ Aug 4 2005, 03:41 PM)
Dear All,
Does anyone can recommand a kit for microbial RNA isolation. I am an absolute greenhorn in terms of working with RNA... I need the RNA for RT-PCR.
I thought the Ambion kit might be good. But then I saw that one is supposed to by such a vortex adapter as well (for a 10min horizontal vortexing step). Our vortexer are ancient and I don't believe any adapter would fit onto it. Does anyone know if there is any way to use the Ambion kit even without buying that adapter?
Best,
Freiberger
Does anyone can recommand a kit for microbial RNA isolation. I am an absolute greenhorn in terms of working with RNA... I need the RNA for RT-PCR.
I thought the Ambion kit might be good. But then I saw that one is supposed to by such a vortex adapter as well (for a 10min horizontal vortexing step). Our vortexer are ancient and I don't believe any adapter would fit onto it. Does anyone know if there is any way to use the Ambion kit even without buying that adapter?
Best,
Freiberger
I have been isolating Microbial RNA with ISOGEN-LS from Gene Japan, the results are very good, why not try it .
Good luck
Wei
-weimin42-
The invitrogen chargeswitch RNA isolation kit will do bacteria
-John Buckels-
There is a new supplier whose kits are cheaper and work well.
I tried and approved :
Clic me
-Mybioforum-