priciples behind nucleic acid isolation - need this for additional lecture information (Aug/02/2005 )

I really need some information on the actual principles behind the following procedures to isolate nucleic acids: alkaline lysis , phenol chloroform extrcation , cesium chloride and silica dioxide.

What actually happens? why it happens ...the chemistry details ...........

thanks

I am not going to answer all your questions, but the basis of CsCl centrifugation is differences in density between, RNA, DNA and protein. CsCl is used because it forms a spontaneous gradient when centrifuged of the right density to separate the above three fractions.

Daniel

DNA sequencing software

hi
try to ask specfic questions.... IT would be easier to answer to a particular point that doing all the chemistry of alkaline lyisi for ex...

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