About MiRanda - (Aug/01/2005 )

hi,
Could anybody tell me that:
1.Can load several miRNAs and a lot of UTRs at one time,respectively?How to achieve it?
2.where can I get the human miRNA pool?
3.Instead of UTRs,can I load exons or introns to analyze?

Thanks for your attention,appreciate greatly for your advice!

1.Can load several miRNAs and a lot of UTRs at one time,respectively?How to achieve it?

You can put mutiple sequences (either miRNA or UTRs) in a fasta file, for example

>sequence1
ggdccgggccgggg
>sequence2
ggsctactataaaaattttttaatata

Use text edit to edit and save the file. Don't use word!

2.where can I get the human miRNA pool?

http://microrna.sanger.ac.uk/cgi-bin/sequences/browse.pl

3.Instead of UTRs,can I load exons or introns to analyze?
miRanda cannot tell whether your seq is UTR or intron, any DNA sequence will do.

Thank you very much!