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haemagglutination assay (help with low titre) - (Aug/01/2005 )

Who has experience with haemagglutionation?

Is it normal that the result in haemagglutination depends rather on the total protein concentration than on actual antibody content?

Trouble shooting welcome!

Thank you.

-Elbo-

The agglutination should be due to the titer of the ab. You should run serial dilutions of your stock(s) ab with your ag sensitized rbcs.

-sgt4boston-