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cloning 60nt Oligos - Help (Mar/08/2002 )

Hello,
I am trying to clone 60nt Oligos to pYes2 plasmid,  who can give me a detail protocol or advice?

Thanks in advance.

Sincerely,

Zhaojun

-zhaojun21-

Hi,
why don't you synthetis two 64 oligos ? You could hybridize them togheter and thus create restriction sites on both sides...
simply ligates into your vector cut with the corresponding Restriction enzymes.
WARP

-warp-