Simple method for lymphocyte extraction - (Jun/24/2005 )
Has anyone got an easy / straightforward / quick method for lymphocyte purification from whole blood ?
-wdavis-
in this article there is a protocol of enzymatic extraction with collagenase. But i can't access the full article so i don't know if it's what you really need
-fred_33-
QUOTE (wdavis @ Jun 24 2005, 09:23 PM)
Has anyone got an easy / straightforward / quick method for lymphocyte purification from whole blood ?
Hello! We are making lymphocytes extraction from whole blood using Lymphoprep from Axis-Shield. It is cheaper than Ficoll and very simple, you could see it in the protocol that I have attached.
-Patri-