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Protein pellet solubilization trouble - (Jun/16/2005 )

i've used a tca precipitation with nadoc and acetone, but after drying i cannot resuspend my pellet with my buffer lysis. i don't know if the problem is tht my samples have been in tca at 4ºc overnight. it's too much?

-bertis-

hi
make an other wash with cold acetone. after this, let dry for at least 30' and resuspend in your lysis buffer, preheated and incubate at 37° with vigourous shaking.
if not working, let mix overnight at 4°

-fred_33-

hi
this topic may help too.

-fred_33-