splice variants - (Jun/08/2005 )

How can i check splice variants?



Thanks!

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Several ways:
1. use ensembl, ensembl ( http://www.ensembl.org/Homo_sapiens/genevi...ENSG00000011304 ) genes give you all splice variant they know, shown here is the link to the PTBP1 protein, which has 3 variants;
2. check alternative splicing database ASD ( http://www.ebi.ac.uk/asd/ );
3. DIY, get all EST/cDNA of your gene, align them with your gene sequence and identify splice variants yourself.

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Thanks!


When you say "your gene" do you mean the gDNA, unmature or mature mRNA?

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When you say "your gene" do you mean the gDNA, unmature or mature mRNA?

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Align EST to genomic sequences.

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If you search for the gene isoforms of human,rat or mouse ,you can use ECgene(http://genome.ewha.ac.kr/ECgene/).

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