CHIP - region - (May/18/2005 )

Hi, I want to CHIP over a whole region of a gene. Do the primers have to have exactly the same Tm and amplify the same length to ensure reliable data? Cheers, Andi

I see no reason for keeping everything the same, but it won't harm if you do. Make sure for every set of primers, the condition is optimized and the amplification is in linear range.