Keratinocyte cultures with serum or serum free media - (May/17/2005 )

can i know suggestions, ideas and opinions about the keratinocyte culture??

should it be cultivated under serum contained media or serum free media??
around what are the seeding densities do vary with the serum free media??

ur suggestions and ideas are very much appreciated! pls! because i'm confusing using the serum free media, but the cells do not show any proliferation. sad..

thanks!!

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In my experience with serum-free media, cell lines that were normally adherent did not adhere, and did not grow well (Cho1.1, H460, 293T); however, cells that would grow in clumps were more clumpy, but grew slowly (HepG2, H157). I do not know what these changes is growth behavior and apparence have on subsequent experiments, but I would guess it varies with cell lines.
Christy

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