Wash cells or not after trypsinization? - (May/03/2005 )
I have grown over forty different adherent cell lines and for most, just adding serum-containing media works great. The finicky cells are usually primary cells, which will die if the trypsin is not removed (eg, HCAEC - endothelial cells).
If you are unsure, compare spinning vs no spinning ... if both survive, go for the quicker alternative 
Usually, I add 2 ml typsin to flask and shake gently and then take out all of typsin, then incubae it at 37 degree for 2 or three minutes. the cells will come off from the flask. Just go ahead to add fresh medium. no wash step.
depends whether I am splitting, banking or harvesting for NAP
I have had a bit of a discussion today, as to whether you will have to spin down and wash cells after trypsinisation or just add medium to the cell / trypsin suspension. I personally have never had any problems by just inactivating the trypsin by adding fresh medium, but would like to hear
your opinions too , before admitting defeat ( TA is always right!
)As AussieUSA says above: (time permitting) there is nothing like a side-by-side comparison of both procedures!
Someone told me that the adherent cells can be lift from the bottom by putting the flask on ice, rather than using Trypsin.
I haven't try it yet. 
Hi
You should wash the cells with 1X PBS and then immediately add serum containing medium to arrest the tripsin action in the cell suspention.
Bye
Hi
You should wash the cells with 1X PBS and then immediately add serum containing medium to arrest the tripsin action in the cell suspention.
Use 0.25 % tripsin for tripsinisation.
Bye
I usually spin down the cells, remove medium with trypsin, and then resuspend with fresh medium.
i usually don't wash after trypsinization...just directly add the medium 