Rt-qPCR: Anti-logged? - (Apr/23/2005 )

It says; "Adjusted average Ct values are used to calculate the average of the biological replicates, and then the delta Ct (Ct target gene – Ct target control) is calculated between those groups. This number is anti-logged to calculate fold change."

What does it mean with anti-logged and how can i make it?


Thanks.

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Dear Friend,
Could you please tell me what real-time machine are you using?

Regards
yong

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Dear yong!

Thank you alot for your replies. It is a Light cycler. As you know i have no clues about Rt-qPCR and i don't know which methods i should use to calculate my expression amounts. The protocol i asked is one i have found in the net and i want to use it for calculating, but i have problem in getting the meaning of it.


Thanks again for your inputs. Greatly appreciated!

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