Rt-qPCR and Microarray - (Apr/20/2005 )

Hello everybody,

I have got the feeling that questions regarding about these two techniques are seldom answered. Does it mean that not many of us have dealt with them? I am currently doing these things and i really need help. Do you know which manufacturers are rapidly and kind in answering these questions?

Thank you all very much.

-indoubt-

QUOTE (indoubt @ Apr 20 2005, 07:23 AM)

Hello everybody,

I have got the feeling that questions regarding about these two techniques are seldom answered. Does it mean that not many of us have dealt with them?

I am currently doing these things and i really need help. Do you know which manufacturers are rapidly and kind in answering these questions?

Thank you all very much.

Applied Biosystems has a very competent and helpful tech support staff and they will give you good advice on qRT PCR. However, it is really up to you to weigh the strenghts and weaknesses of each technique and analysis method. Microarray analysis and validation of those results is extremely tricky and complicated.

-turnerm-

Hi

Try http://www.hgmp.mrc.ac.uk/GenomeWeb/nuc-genexp.html

Also,
DNA microarrays : a molecular cloning manual / edited by David Bowtell and Joseph Sambrook.
Cold Spring Harbor, N.Y.

Check out Stanford University's site also!

What kind of microarray are you planning to use?

SV

-SVTX-