CO2 must be 5% or 10%? - (Apr/18/2005 )

Hi,

I was told to culture different cells in 5% or 10% CO2, is it so important? I think most of the cell lines will be OK in 5%, right?

Thank you!

-jxy-

QUOTE (jxy @ Apr 18 2005, 08:30 PM)

Hi,

I was told to culture different cells in 5% or 10% CO2, is it so important? I think most of the cell lines will be OK in 5%, right?

Thank you!

I think the amount of Co2 depends on the amount of NaHCo3 in your medium. The higher the bicarbonate the higher the Co2 you need.

-indoubt-

hi
usually 5%CO2 is used. But this should be available on your manufacturer's website.

fred

-fred_33-