seeding volume - (Mar/25/2005 )

What are the typical seeding volumes you have for these T-25, T-75, T-150 flasks?

THanks for any ideas.

-justwonder-

QUOTE (justwonder @ Mar 25 2005, 12:10 PM)

What are the typical seeding volumes you have for these T-25, T-75, T-150 flasks?

T-25: 6ml, T-75: 15ml, T-150: 30-40 ml according to:
McAtier, J.A. and Daview, J., Basic Cell Culture. A Practical Approach., Davies, J.M. (Ed) p. 153 (Oxford University Press Inc, New York, 1998).

THanks for any ideas.

-diana-

Hi there,

Do you mean, how much volume of media can you have in T25, T75s?

If so, then you are right.

T25 - about 5mls
T75 - about 10 - 15 mls
T-150 - about 20-40 mls.

-Maria-

The stated values are good volume ranges for adherent cells however, if you are asking for seeding volumes for suspension cultures try ...

T25: 4-12 ml
T75: 8-40 ml (can do 50 ml if required)
T150: 15-100 ml

with cells at 1 x 10e5 to 2 x 10e5 cells/ml.

-AussieUSA-