Autoclaving and microwaving - (Mar/23/2005 )

Should i put on the lids to my medium bottles when i autoclave them?

Should i cap my agar solution bottle when i microwave it?



Thanks.

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Yes to both questions. But keep the lids loose so that the air and steam can freely go out, and do not blow your bottles.
And do not fill your bottles more than 80% if you do not want to lose too much medium when it boils over

HTH

Patrick

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When the bottles don't have caps, can i cover them with folie and autoclave them or would this damage the folie?



Thanks.

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Aluminium foil is OK. You do not even need to loosen it, it just pops out if the pressure too high. In fact I usually tape the foil with a loose autoclave tape to the bottle, so I do not need to look for it inside the autoclave afterwards.

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