Using Glycogen as a carrier in DNA Extraction - Low DNA Extraction (Mar/22/2005 )
I am trying to extract DNA from samples that have a low amount of cells present, and have read in protocols to add glycogen, LPA, tRNA, etc, to improve yields. How exactly does glycogen work as a carrier in DNA precipitation to give greater yields?
Many thanks
-Miles-
I'm not 100% sure, guess it binds to the DNA present?
-Maria-
check these references:
Tracy S Prep. Biochem 1981; 11:251-268
Helms, C et al DNA 1985: 4 39-49
-pcrman-