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How to insert 10His-TEV cassette upstream of coding sequence - (Aug/10/2002 )

Colleagues, could you share you approaches/experience in inserting 10xHis--TEV cassette upstream of coding sequence for a protein to be expressed (in yeast)?

-Yakov-

Try out using the Ex-Site-Mutagenesis-Kit from Stratagene, if there is no targeting-sequence (for example for mitochondrial transit)

-Wotan-