How to insert 10His-TEV cassette upstream of coding sequence - (Aug/10/2002 )
Colleagues, could you share you approaches/experience in inserting 10xHis--TEV cassette upstream of coding sequence for a protein to be expressed (in yeast)?
-Yakov-
Try out using the Ex-Site-Mutagenesis-Kit from Stratagene, if there is no targeting-sequence (for example for mitochondrial transit)
-Wotan-