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The significance of the 260/230 ratio for nucleic acid purity - (Dec/03/2004 )

hi,
what is the significance of 260/230nm spectroscopic reading of DNA

-chandima-

hi
i summarize what is on this web page from Hillary Luebbehusen
http://www.bcm.edu/mcfweb/Modified%20260_230%20for%20pdf.pdf

signifiance of 260/230 ratio is a measure to evaluate the purity of your prep regarding the organic compounds in solution (phenol, trizol and others aromatics like these).
The autir recommend strongly to use preps with a ratio 260/230 greater than 1.8

Fred_33

-fred_33-