ligation and transformation problems! Urgent! - (Nov/30/2004 )

I re-double cut the vector in a relative short time 1.5h.
I change the ratio of insert to vector. I usually set up the ligation based on the mass ratio of 3:1. But this time I change the mass ratio to molar ratio. I remember someone tells me that too much insert will inhibit the ligation.

ng of insert=ng of vector*(kb of insert/kb of vector)*(3/1)

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