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Ovalbumin - (Nov/30/2004 )

Hi
Do someone know why is OVA used like an Ag???
Where I can find this information??? Any article???
Thanks a lot

-Microbiologa-

Hi Microbiologa,
The reason i use OVA as an antigen is because i have mice with a transgenic T cell receptor for it (OT-1, OT-2 and DO11.10 mice). This way is is easier to study APC-T cell interaction because 80% of the T cells in these mice are specific for OVA (in an allogeneic reaction like C57Bl6 to Balb/c this is 1-5%). I don't know if there are other reasons to use OVA. And for articles about it, just search pubmed for it and you will find plenty.

Ilse2you

-ilse2you-

Low endotoxin Ovalbumin

Commercially available stocks of OVA are heavily contaminated with LPS.
Ovalbumin is used in conjugo-immuno-determinations and drug and pharmaceutical processing. Also it is often used as a non-relevant carrier protein in ELISA antibody titer screens or as blocking buffer. “However, … , commercially available stocks of proteins, such as HEL, BSA, or OVA, are heavily contaminated with LPS, … .”
[J. Immunol., Jun 1999; 162: 6552 - 6561.]
Therefore not only in biopharma production but also in R&D the removal of endotoxin from ovalbumin solution is of great importance.

Profos offers an application note which summarized general information on ovalbumin. Answers are given to questions like:

Posttranslational Modifications
Physical and chemical properties
detailed protocol for removal of endotoxin from ovalbumin with EndoTrap

For more details please contact Profos AG (Germany).

-EndoTrap-