IPTG induction - (Dec/22/2008 )
I wnat to ask you that its good to cool the media when it reach OD 6.0 for induction or induction can be done at the same temerature??????
-julebo-
QUOTE (julebo @ Dec 22 2008, 02:02 PM)
I wnat to ask you that its good to cool the media when it reach OD 6.0 for induction or induction can be done at the same temerature??????
The same temperature is fine. I just add my IPTG directly to the culture in the incubator.
-Cheamps-