help with grant writing - (Dec/17/2008 )
Iam trying to write a grant on chemoprevention . its basically about looking at the molecular mechanism of antiproliferative effects of some compound on cancer cells
Basically the mechanisms that i would like to look at are anti inflammatory effect , cell cycle , apoptosis and so on.
The portion I'am having trouble with is with the interpretation and pitfall section.
for eg if i write in one of my aims that i want to see the effect of my compound on cell cycle modulation/or apoptosis. and this would be done by flow cytometry and western blot analysis of proteins involved i.
iam not sure what to write in the interpretation and pitfall section for thiese aim. If someone can please help that would be great
thanks in advance
Things you may want to consider are: What are you going to do if your hypothesis is wrong (the drug doesn't have any effect (antigrowth, cell cycle apotosis, etc)) and if it has the effects but only at higher than physiological concentrations? Are the doses you proposed appropirate?
thanks for your response. i already have some preliminary data which reveals the antigrowth effects. and also cell cycle modulation and apoptosis by flow cytometry. so the doses at which i have already seen some effect would be proposed in the study.
In that case you can just say something like we expect to be able to obtain xxx results which are supported by our preliminary data, the feasibility has been demonstrated by our preliminary data, we don't expect any technical difficulties, blah, blah,
Because this experiment is quite straight forward, there is no much to write about pitfalls, alternative strategies...
Because this experiment is quite straight forward, there is no much to write about pitfalls, alternative strategies...
thank you very much, that makes sense