Trizol in freezer - (Dec/09/2008 )

Hi all,

I have recently started using Trizol to dissolve my cell pellets, in order to isolate DNA/RNA/protein later. My colleague said it was ok to store dissolved pellet in 1 ml Trizol at -20. However, when I checked my tubes today, most were frozen solid, but a couple of them were still liquid (more than 5 days in freezer). Any idea how this can happen?

Thanks for your suggestions.

-DavidJ-

Hi there

I always store my "trizol stocks" (ie: cell pellet dissolved in trizol) at -80 (not -20!). Works brilliantly -20 freezers seem to have 'hot' spots where things don't freeze.
Clare

QUOTE (DavidJ @ Dec 9 2008, 04:25 PM)

-Clare-

QUOTE (DavidJ @ Dec 9 2008, 10:25 AM)

I have recently started using Trizol to dissolve my cell pellets, in order to isolate DNA/RNA/protein later. My colleague said it was ok to store dissolved pellet in 1 ml Trizol at -20. However, when I checked my tubes today, most were frozen solid, but a couple of them were still liquid (more than 5 days in freezer). Any idea how this can happen?

Thanks for your suggestions.

If you would like to do RNA isolation, you should put TRIzol dissolved samples in -80 for better RNA quality.
I think -20 is at the point TRIzol is about to freeze or just froze, so dependent on what do you have in your samples, some unfrozen tubes are possible.

Again, for better sample preparation later, store your TRIzol tubes in -80.

-wuxx0153-