Developing, Validating and Standardizing ELISA - Developing, Validating and Standardizing ELISA (Nov/25/2008 )
Hello all,.
I am making a general protocol for Developing, Validating and Standardizing direct ELISA for any type of antibody. The protocol I am typing out includes all the type of precautions and how to determine the right plate, coating concentration, right serum dilution, finding right secondary antibody titer and the whole deal in ELISA.
Would like to know if anyone has come across something similar to this or if anyone has any any written up stuff from where I can start off. Once done would like to put it up here on protocol- online for anyone to download.
thekid
I am making a general protocol for Developing, Validating and Standardizing direct ELISA for any type of antibody. The protocol I am typing out includes all the type of precautions and how to determine the right plate, coating concentration, right serum dilution, finding right secondary antibody titer and the whole deal in ELISA.
Would like to know if anyone has come across something similar to this or if anyone has any any written up stuff from where I can start off. Once done would like to put it up here on protocol- online for anyone to download.
thekid
You can check out these ELISA Protocols to assist you in developing one great overarching protocol!
Once you finish that off, would you like to have a go at the unified field theory? It's been annoying people for some time now. 
Wow! Do you know what you're getting yourself into? What level of validation? What degree of standardisation? Research only, or diagnostic as well?