anaerobic / microaerophilic, are they really different ? - (Nov/21/2008 )

Hi all

I am trying to growth a microaerophilic bacteria, however, I don't have any anaerobic jar or anaerobic gas pack.
how can I prepare 5 ml liquid culture easily ? without any anaerobic gas chamber ?

is that possible to make a burning candle inside the culture tube to remove oxygen ? (sound stupid....)

Thx very much

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Have you tried just growing it in a mostly full tube with the lid on tightly? I've grown Borrelia, which is supposed to be microaerophilic and grown in a CO2 incubator, just in tubes with the lid on and they grow just fine. In fact, I think many Borrelia labs don't have CO2 incubators at all.

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If you don't shake them you should be able to out a layer of mineral oil on top of it.

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I grow 10 ml culture of S. pyogenes by closing the cap very tightly and that works perfectly. I only use anaerobic pouches or jars with gas pack for my plates. And I don't have CO2 incubator either.

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thank you very much,
I didn't try filling the tube full to remove most air inside.

However, should I remove dissolved oxygen in the solution too ? e.g. ultrasound or applying vacuum ?

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you could sparge the solution with N2 gas filtered through .2 micron

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Thx you very much

actually, I am trying to grow my poor magnetic bacteria (M magnetotacticum), I tried all you guys suggestion, but it never growth.......

I followed the ATCC instruction but nothing happen....Is that bacteria really difficult to growth ???

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